Laboratory for electron microscopy

Laboratory for electron microscopy

SEM and TEM analysis

The laboratory for electron microscopy is fully equipped for both Scanning and Transmission Electron Microscopic analyses (SEM and TEM respectively):

  • TEM analyses produce images that have higher magnification and greater resolution.
  • SEM analyses produce three-dimensional images which provide more information about the shape of features and location of features relative to each other.  Both biological and non-biological samples can be analysed with a SEM, in either low or high vacuum mode, using either secondary of back-scattered electrons.

For these analysis methods both paraffin and frozen sections can be made. These sections can be stained using routine staining protocols (e.g. dichrome staining such as haematoxylin-eosin, trichrome staining such as Masson staining,...) but more often, an immunohistochemical staining is applied, allowing the visualisation of a particular molecule/structure.

Equipment

The following equipment and software are present in this laboratory and can be rented (reservation and training required):

  • Three-headed Discussion Light Microscope (OLYMPUS, BX53)
  • Fully motorized Fluorescence Microscope (OLYMPUS, BX61) with high resolution Camera (OLYMPUS, DP73)
  • Stereo Microscope (OLYMPUS, SZX7) with Camera (OLYMPUS, SC100)
  • Inverted Microscope (OLYMPUS, CKX41) with Camera (OLYMPUS, SC50)
  • CellSens Dimension software (Olympus) (image analysis)
  • Stepanizer software (stereology)
  • Amira software (3-D reconstruction)

Additionally, all kind of on-demand services can be performed, from simple embedding tissue into paraffin to complete microscopic analysis (from tissue to qualitative or quantitative result).

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