Laboratory for light microscopy

Laboratory for light microscopy

Light microscopy

The laboratory for light microscopy is  fully equipped for making both paraffin and frozen sections. These sections can be stained using routine staining protocols (e.g. dichrome staining such as haematoxylin-eosin, trichrome staining such as Masson staining,...) but more often, an immunohistochemical staining is applied, allowing the visualisation of a particular molecule/structure.

Subsequently, the stained tissue sections can be analysed, either qualitatively or (semi-) quantitatively:

  • A qualitative analysis is based on the experience of the observer.
  • For a (semi-)quantitative analysis, software is used. In this case, there are two options, namely fully automated image analysis and stereology.
  • Instead of a qualitative or quantitative analysis, a 3-dimensional reconstruction can be made based on a set of serial sections.
  • Recently laser capture microdissection can be performed on a set of sections, allowing the dissection of a group of cells out of a section. These isolated cells can be used for DNA or mRNA isolation, after which a qualitative or (semi-)quantitative gene analysis can be executed by PCR using gene specific primers.

Equipment

The following equipment and software are present in this laboratory and can be rented (reservation and training required):

  • Three-headed Discussion Light Microscope (OLYMPUS, BX53)
  • Fully motorized Fluorescence Microscope (OLYMPUS, BX61) with high resolution Camera (OLYMPUS, DP73)
  • Stereo Microscope (OLYMPUS, SZX7) with Camera (OLYMPUS, SC100)
  • Inverted Microscope (OLYMPUS, CKX41) with Camera (OLYMPUS, SC50)
  • CellSens Dimension software (Olympus) (image analysis)
  • Stepanizer software (stereology)
  • Amira software (3-D reconstruction)

Additionally, all kind of on-demand services can be performed, from simple embedding tissue into paraffin to complete microscopic analysis (from tissue to qualitative or quantitative result).

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